Life Science

z7413WP - Demystifying Accuracy in Life Sciences Instrumentation

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To achieve the required accuracy and precision in a given application it is important to understand resolution and repeatability Both are important to matching syringe pump performance to application-specific requirements. RESOLUTION Resolution refers to the quantity of discrete, measurable increments of an instrument. A scale that measures in grams has a higher resolution than one that measures in kilograms. Similarly, a syringe pump with 48,000 steps per stroke has a higher resolution than a pump with 12,000 steps per stroke, because there are more discrete locations at which the pump can stop. Do not confuse higher resolution with better accuracy! The 12,000-step pump might be more accurate or less accurate than the 48,000-step pump. Resolution says how small a step you can take, but it does not say whether the step will go where you expect it to go. REPEATABILITY Repeatability is how consistently the same actions will produce the same results. The key concept is the same actions – the starting point, ending point, and all conditions in between are identical each time the action is performed. In the specification of syringe pumps, the most common scenario is to set the syringe to its top-of-stoke, aspirate a full stroke of fluid, and then dispense the full stroke back to top-of-stroke. While a valid approach, this methodology does not account for any variation along the barrel of the syringe. As an alternative, the syringe may begin at some point, aspirate a small, fixed amount (for example, 1/10th of the syringe volume) and then dispense the amount just aspirated. This offers a more stringent evaluation of repeatability as it is closer to actual operation in many applications and any variation in the syringe barrel will impact the result. When assessing repeatability, one should remember that if the conditions are changed, the results might not be the same. For example, it the syringe positions at the beginning and end of the stroke are not the same beginning and end positions every time, the results will not be the same either. Resolution and Repeatability Fully customisable Zero dead volume design Customisable solenoid manifold for intelligent fluid pathway Dispenses up to 8 samples simultaneously 04 white paper

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